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200182

Sigma-Aldrich

Nitrazine Yellow

indicator grade, Dye content 85 %

Sinonimo/i:

2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow

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About This Item

Formula empirica (notazione di Hill):
C16H8N4Na2O11S2
Numero CAS:
5423-07-4
Peso molecolare:
542.36
Colour Index:
14890
Numero CE:
226-549-8
Numero MDL:
MFCD00003938
Codice UNSPSC:
12171500
ID PubChem:
24851961
NACRES:
NA.47

Grado

indicator grade

Saggio

>90.0% (HPLC)

Stato

powder

Composizione

Dye content, 85%

Solubilità

H2O: 1 mg/mL

λmax

586 nm

ε (coefficiente d’estinzione)

≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;
XBLIFEQTVVSTIM-QIKYXUGXSA-L

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Applicazioni

Nitrazine Yellow has been used in dye-protein binding assay.[1]

Azioni biochim/fisiol

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye.[2] It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy.[2][3] Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.[4]

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
MKCD1402
MKBQ6886V
MKBG8083V
MKBD9527V
36296KJ

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Gilbert G G Donders et al.
Infectious diseases in obstetrics and gynecology, 2010, 504972-504972 (2010-10-19)
Accessing vaginal pH is fundamental during gynaecological visit for the detection of abnormal vaginal flora (AVF), but use of pH strips may be time-consuming and difficult to interpret. The aim of this study was to evaluate the VS-SENSE test (Common
Structural characterisation of Nitrazine Yellow by NMR spectroscopy
Viscardi G
Dyes and Pigments, 27, 87- 95 (2003)
T Kishida et al.
European journal of obstetrics, gynecology, and reproductive biology, 58(1), 67-72 (1995-01-01)
We have developed a new anti-AFP monoclonal antibody kit which is easier to use than other examination methods for detecting AFP in the leaked amniotic fluid. In this study, we investigate the clinical value of this test in the diagnosis
"Fig leaf" ferning and positive Nitrazine testing: semen as a cause of misdiagnosis of premature rupture of membranes.
J A McGregor et al.
American journal of obstetrics and gynecology, 151(8), 1142-1143 (1985-04-15)
O Mäki-Petäys et al.
Acta veterinaria Scandinavica, 32(1), 123-129 (1991-01-01)
The accuracy of portable pH meters and the nitrazine yellow method was compared with the reference method by determining the pH of 74 beef and 96 pork muscles. The pH was measured directly from the muscle. The results showed statistically
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