HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
rat plasma spiked with provastin and atorvastin at 5 and 100 ng/mL
SPE tube/cartridge
Supel-Select HLB SPE, 30 mg/1 mL (54181-U)
condition
0.5 mL methanol:acetonitrile (1:1, v/v); 0.5 mL DI water
sample addition
0.5 mL
washing
0.5 mL 5% methanol
elution
0.5 mL methanol:acetonitrile (1:1, v/v)
column
Ascentis Express C18, 5 cm x 2.1 mm, 2.7 μm particles (53822-U)
eluate post-treatment
evaporate to dryness with nitrogen at 37 °C, reconstitute in 0.5 mL DI water
mobile phase
0.1% acetic acid diluted in 60% methanol
flow rate
0.2 mL/min
column temp.
35 °C
detector
ABI 3200 QT; ESI(+), MRM (423.3/321.3, 423.3/101.0, 557.3/397.2 and 557.3/453.4)
injection
5 μL
Description
Informations légales
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC