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HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

Z777033

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel

Composant de phase mobile

Réf. du produit
Description
Tarif

452289

Acide phosphorique

≥85 wt. % in H2O, ≥99.999% trace metals basis

Étalon

Réf. du produit
Description
Tarif

M1882

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

U0750

Uracile

≥99.0%

T1001

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

I4131

IgG from rat serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

05470

Albumine de sérum bovin

lyophilized powder, crystallized, ≥98.0% (GE)

CONDITIONS

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm (Z777033)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid

flow rate

0.1 mL/min

pressure

173 psi (12 bar)

column temp.

25 °C

detector

UV, 215 nm

injection

4 μL

sample

protein standards

Description

Remarque sur l'analyse

Due to the low diffusion coefficents of proteins, optimal flow in size exclusion chromatography is often very low. However, the disadvantage is long run times. This example demonstrates both features.

Informations légales

Zenix is a registered trademark of Sepax Technologies

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS