HPLC Analysis of Phenylsuccinic Anhydride Enantiomers on Astec^® (R,R) P-CAP^™-DP

column

Astec (R,R) P-CAP-DP, 25 cm x 4.6 mm I.D., 5 μm particles (35024AST)

mobile phase

[A] acetonitrile; [B] methanol; [C] trifluoroacetic acid; (99:1/0.1 (A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

1 μL

sample

1 mg/mL in methanol