HPLC Analysis of Insulin, Bovine on Discovery^® C8

column

Discovery C8, 5 cm × 4.6 mm I.D., 5 μm particles (59353-U)

mobile phase

50 mM sodium phosphate, monobasic, 50 mM sodium sulfate, pH 3.0 (w/phosphoric acid), (A) +5% acetonitrile, (B) +50% acetonitrile

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm