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HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection)

HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection) application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

53916-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

Étalon

Réf. du produit
Description
Tarif

C1778

Caféine

Sigma Reference Standard, vial of 250 mg

S1626

Acide sorbique

99.0-101.0% anhydrous basis

47862

Pyridoxine Hydrochloride (B6)

analytical standard

47865-U

Nicotinamide (niacinamide)

analytical standard

47861

Riboflavine (B2)

analytical standard

8S61336

Benzoic acid solution

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)

mobile phase

[A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA

flow rate

0.6 mL/min

pressure

1146 psi (79 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Energy Drink Diluted 1:9 in acetonitrile

Description

Remarque sur l'analyse

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in caffeinated energy drinks.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

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