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53567-U

Supelco

Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 2.1 mm, HPLC Column

Synonyme(s) :

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

Code UNSPSC :
41115700
eCl@ss :
32110501
Nomenclature NACRES :
SB.52

product name

Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

Matériaux

stainless steel column

Agence

suitable for USP L43

Gamme de produits

Ascentis®

Caractéristiques

endcapped

Fabricant/nom de marque

Ascentis®

Conditionnement

1 ea of

Paramètres

60 °C temp. range
600 bar max. pressure (9000 psi)

Technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × D.I.

5 cm × 2.1 mm

Superficie

135 m2/g

Impuretés

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Groupe de la matrice active

PFP (pentafluorophenyl) phase

Taille des particules

2.7 μm

Dimension de pores

90 Å pore size

pH de fonctionnement

2-8

Application(s)

food and beverages

Technique de séparation

reversed phase

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Description générale

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore

Articles

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Protocoles

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

Chromatograms

application for HPLC

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