ZRB2282

Anti-DNAJB9 Antibody, clone 1D16-K ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : ER-resident protein ERdj4, Endoplasmic reticulum DNA J domain-containing protein 4, Microvascular endothelial differentiation gene 1 protein, UNQ743/PRO1471
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1D16-K, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 25.52 kDa; observed mol wt ~25 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human, mouse, rat
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Internal
• Numéro d'accès Protein ID: NP_036460
• Numéro d'accès UniProt: Q9UBS3
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... DNAJB9(4189)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1D16-K is a ZooMAb^® rabbit recombinant monoclonal antibody that detects DNAJB9. It targets an epitope within 23 amino acids from the C-terminal half of the protein.

Immunogène

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human DNAJB9.

Application

Quality Control Testing

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DNAJB9 in mouse kidney tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DNAJB9 in rat kidney tissue lysates.

Affinity Binding Assay: The binding affinity of this antibody to DNAJB9 peptide was at least one hundred thousand-fold (100,000X) higher than that obtained with the non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DNAJB9 in human kidney and mouse kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

DnaJ homolog subfamily B member 9 (UniProt: Q9UBS3; also known as Endoplasmic reticulum DNA J domain-containing protein 4, ER-resident protein ERdj4, Microvascular endothelial differentiation gene 1 protein) is encoded by the DNAJB9 (also known as MDG1, UNQ743/PRO1471) gene (Gene ID:4189) in human. DNAJB9 belongs to the DnaJ/Hsp40 protein family, which is involved in chaperone-mediated protein folding and cellular stress responses. DNAJB9 consists of various functional domains, including a J domain, which is responsible for interacting with Hsp70 chaperones, and a cysteine-rich domain implicated in protein-protein interactions. The expression profile of DNAJB9 varies across different tissues and cell types. It is expressed in various organs, including the brain, heart, liver, and kidney. DNAJB9 expression levels can be regulated under different physiological and pathological conditions, such as during cellular stress or in certain disease states. Increased expression of DNAJB9 has been observed in certain brain regions affected by diseases. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable