ZRB2098

Anti-SRF Antibody, clone 1H14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Serum response factor
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.46

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1H14, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 51.59 kDa; observed mol wt ~60 kDa
• Produit purifié par: using Protein A
• Espèces réactives: rat, mouse, human
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal half
• Numéro d'accès Protein ID: NP_001278930
• Numéro d'accès UniProt: P11831
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... SRF(6722)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1H14 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Serum response factor (SRF). It targets an epitope within 21 amino acids from the C-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal half of human serum response factor (SRF).

Application

Quality Control Testing
Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected serum response factor (SRF) in HeLa cell nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected serum response factor (SRF) in Rat brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected serum response factor (SRF) in Human kidney tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected serum response factor (SRF) in HeLa, HepG2, and NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Serum response factor (UniProt: P11831; also known as SRF) is encoded by the SRF gene (Gene ID: 6722) in human. SRF is a transcription factor that binds to a serum response element (SRE) associated with a variety of genes, including immediate early genes such as c-Fos, FosB, JunB, Egr-1 and -2; neuronal genes such as nurr1 and nur77. It binds to DNA as a dimer and its DNA binding region is localized in amino acids 133-222. By regulating the expression of several genes, it controls cell growth and differentiation, neuronal transmission, and muscle development. Together with Myocardin Related Transcription Factor A (MRTFA) transcription coactivator, it controls the expression of genes regulating cytoskeleton development, morphogenesis, and cell migration. It is also required for cardiac differentiation and maturation. SRF is shown to be activated by a variety of agents, including serum, lysophosphatidic acid, lipopolysaccharide, 12-O-tetradecanoylphorbol-13-acetate (TPA), and cytokines. Keratinocytes in normal human and mouse skin are reported to express high levels of SRF, but its expression is strongly downregulated in the hyperproliferative epidermis of wounded and psoriatic skin. Loss of SRF is known to disrupt epidermal homeostasis. Mice lacking SRF in cardiac tissue display lethal cardiac defects between E10.5 and E13.5. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hu, X., et al. (2019). Nat. Commun. 10(1); 1695; Koegel, H., et al. (2009). J. Clin. Invest. 119(4); 899-910; Parlakian , A., et al (2004). Mol. Cell Biol. 24(12); 5281-5289; Chai, J., and Tarnawski, AS. (2002). J. Physiol. Pharmacol. 53(2); 147-157).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable