ZRB1255

Anti-CD8β Antibody, clone 1H21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1H21, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 23.72 kDa; observed mol wt ~34 kDa
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): ELISA: suitable; immunocytochemistry: suitable
• Isotype: IgG
• Séquence de l'épitope: Extracellular domain
• Numéro d'accès Protein ID: NP_004922.1
• Numéro d'accès UniProt: P10966
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 1H21 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects human T-cell surface glycoprotein CD8 beta chain (CD8β). It targets an epitope within 13 amino acids from the extracellular domain.

Immunogène

KLH-conjugated linear peptide corresponding to 13 amino acids from the extracellular domain of human T-cell surface glycoprotein CD8 beta chain (CD8β).

Application

Quality Control Testing

Evaluated by Western Blotting in PBMC cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD8β in peripheral blood mononuclear cell (PBMC) lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD8β in human thymus and human spleen tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD8β in human thymus tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CD8β peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Flow Cytometry Analysis: 1 βg from a representative lot detected CD8β in one million Hut78 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD8β in Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CD8β, clone 1H21 ZooMAb^®, Cat. No. ZRB1255, is a recombinant Rabbit monoclonal antibody that targets CD8β and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Description de la cible

T-cell surface glycoprotein CD8 beta chain (UniProt: P10966; also known as CD8b) Is encoded by the CD8B (also known as CD8B1) gene (Gene ID: 926) in human. CD8β is a single-pass type I membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-170), a transmembrane domain (aa 171-191), and a cytoplasmic domain (aa 192-210). Eight different isoforms of CD8β have been described that are generated by alternative splicing. Isoforms 1,3,5,6,7,8 are expressed in both thymus and peripheral CD8+ T cells. For efficient cell surface expression, it requires the CD8a as a partner and the heterodimer CD8a/CD8β localizes to lipid rafts due to CD8β cytoplasmic tail palmitoylation. In T-cells, it functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. It interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1