ZMS1120

Anti-Laminin α2 80 kDa Antibody, clone 5H2 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 5H2, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Produit purifié par: using protein G
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rabbit, monkey
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès Protein ID: NP_001073291.1
• Numéro d'accès UniProt: P24043
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 5H2 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Laminin 2. It targets an epitope within the C-terminal region.

Immunogène

80 kDa fragment from the C-terminal region of human Laminin 2.

Application

Quality Control Testing

Evaluated by Western Blotting in Human heart tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Laminin 2 in Human Heart tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Laminin 2 in human heart and human cerebral cortex tissue sections.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Laminin 2 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Laminin subunit alpha-2 (UniProt: P24043; also known as Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain) is encoded by the LAMA2 (also known as LAMM) gene (Gene ID: 3908) in human. Laminins are a family of heterotrimeric extracellular glycoproteins consisting of α, β, and γ chains that are bound to each other by disulfide bonds into a cross-shaped molecule comprising one LONG^® and three short arms with globules at each end.  Thus far five α, three β, and three γ subunits are known, which assemble into at least 15 different laminin molecules.  Laminin α2 is a glycoprotein with multiple EGF-like domains and is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off. It is expressed in multiple tissues, including placenta, striated muscle, cardiac muscle, pancreas, lung, spleen, skin, bone, and some regions of the brain. Laminin α2 binds to cells via a high Affinity^™ receptor and mediates the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.  Mutations in LAMA2 gene have been linked to Merosin-deficient congenital muscular dystrophy 1A (MDC1A) and limb-girdle muscular dystrophy that results in muscle weakness and atrophy.  This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, X., et al. (1996). J. Biol. Chem. 271(44); 27664-27669; Ehrig, K., et al., (1990). Proc. Natl. Acad. Sci. USA. 87(9); 3264-3268).

Forme physique

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

Affinity is a trademark of Mine Safety Appliances Co.

LONG is a registered trademark of Repligen Sweden AB

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable