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Y1771

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

Synonyme(s) :

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements

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About This Item

Numéro MDL:
MFCD01095679
Code UNSPSC :
41106212
Nomenclature NACRES :
NA.85

Niveau de qualité

200

Forme

powder

Technique(s)

transformation: suitable

Application(s)

microbiology

Température de stockage

room temp

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Application

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

  • the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
  • Yarrowia lipolytica PO1f strain positive transformants
  • CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Autres remarques

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Notes préparatoires

Dissolve 1.46 g of supplement per liter of medium.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

G7021

D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A5306

Gélose, for molecular biology

Y1501

Yeast Synthetic Drop-out Medium Supplements, without uracil

Y2001

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan and uracil

Y0626

Base azotée pour levures sans acides aminés, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1896

Yeast Synthetic Drop-out Medium Supplements, without lysine

Y2021

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine

Y2146

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, and tryptophan

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H318

Conseils de prudence

P280 - P305 + P351 + P338

Classification des risques

Eye Dam. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Emil D Jensen et al.
Bio-protocol, 12(5), e4346-e4346 (2022-05-21)
Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants with improved traits from vast mutated sequence spaces.
Paul Swietalski et al.
Yeast (Chichester, England), 39(3), 230-240 (2021-10-15)
Orotic acid (OA) is an intermediate of the pyrimidine biosynthesis with high industrial relevance due to its use as precursor for production of biochemical pyrimidines or its use as carrier molecule in drug formulations. It can be produced by fermentation
Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps

Protocoles

Yeast Drop Out

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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