SRP6420

Kallikrein-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonyme(s) : KLK1, KLKR, Klk6, hK1
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Étiquette/Marqueur: 6-His tagged (C-terminus)
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: calculated mol wt 27.9 kDa; observed mol wt 40-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Pro 19 is the predicted N-terminal.)
• Conditionnement: pkg of 10 μg
• Numéro d'accès UniProt: P06870
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... KLK1(3816)

Description

Description générale

Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular Kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Forme physique

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable