SRP6133

Ubiquitin-K48 human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥85% (SDS-PAGE)
• Forme: liquid
• Poids mol.: 8.732 kDa
• Conditionnement: pkg of 500 μg
• Numéro d'accès NCBI: P62979
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... RPS27A(6233)

Description

Description générale

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines, except at position 48. This molecule, therefore, can only form polyubiquitin chains of K48 linkage type. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

Forme physique

4 mg/mL in 50 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol.

Notes préparatoires

Centrifuge the vial prior to opening.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable