SRP5012
CDK9/CyclinK, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonyme(s) :
C-2k, CCNK, CDC2L4, CPR4, MGC9113, PITALRE, TAK
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produit recombinant
expressed in baculovirus infected Sf9 cells
Gamme de produits
PRECISIO® Kinase
Pureté
≥70% (SDS-PAGE)
Forme
buffered aqueous glycerol solution
Activité spécifique
15-21 nmol/min·mg
Poids mol.
~67 kDa (CyclinK)
~68 kDa (CDK9)
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... CCNK(8812) , CDK9(1025)
Description générale
CDK9/CyclinK, active, GST tagged human comprises recombinant full-length human CDK9 and cyclinK proteins co-expressedin Sf9 insect cells with an N-terminal GST tag. Cyclin-dependent protein kinase 9 (CDK9)/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is a component of the multiprotein complex mitogen-activated protein kinase 7 and transcription elongation factor β (TAK/P-TEFβ).
Actions biochimiques/physiologiques
CDKs are closely related to yeast CDk1, cdc28 and cdc2 and arean important regulators of the cell cycle. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the human immune deficiency virus 1 (HIV-1) transactivating regulatory protein (Tat) protein, which suggests a possible involvement of this protein in AIDS.
Forme physique
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Notes préparatoires
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Informations légales
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Ranadip Mandal et al.
Cellular and molecular life sciences : CMLS, 79(12), 597-597 (2022-11-19)
Cervical cancer is the fourth most frequently diagnosed and fatal gynecological cancer. 15-61% of all cases metastasize and develop chemoresistance, reducing the 5-year survival of cervical cancer patients to as low as 17%. Therefore, unraveling the mechanisms contributing to metastasis
Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation
Kim YK, et al.
Molecular and Cellular Biology, 22(13), 4622-4637 (2002)
The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription
Yang Z, et al.
Nature, 414(6861), 317-322 (2001)
Z Yang et al.
Nature, 414(6861), 317-322 (2001-11-20)
The human positive transcription elongation factor P-TEFb, consisting of a CDK9/cyclin T1 heterodimer, functions as both a general and an HIV-1 Tat-specific transcription factor. P-TEFb activates transcription by phosphorylating RNA polymerase (Pol) II, leading to the formation of processive elongation
F Bullrich et al.
Cancer research, 55(6), 1199-1205 (1995-03-15)
Orderly progression through the cell cycle requires sequential activation and inactivation of cyclin-dependent kinases (cdks). This is achieved in part through the association of cdks with positive regulators called cyclins and inactivation of cyclin-cdk complexes by a rapidly growing number
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