SRP3309
GDNF human
recombinant, expressed in E. coli, Animal-component free, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonyme(s) :
ATF-1
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in E. coli
Pureté
≥98% (HPLC)
≥98% (SDS-PAGE)
Forme
lyophilized
Puissance
≤0.1 μg/mL
Poids mol.
30.0 kDa
Conditionnement
pkg of 10 μg
Impuretés
endotoxin, tested
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... GDNF(2668)
Description générale
Glial cell derived neurotrophic factor (GDNF) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The gene encoding this protein is localized on human chromosome 5p13.2. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Application
Glial cell derived neurotrophic factor (GDNF) has been used as a supplement in dorsal root ganglion (DRG) culture medium to study the effect of growth factors on neuron viability, neurite outgrowth and arborisation. (3)
Actions biochimiques/physiologiques
Glial cell derived neurotrophic factor (GDNF) signals through a multicomponent receptor system, composed of a RET and one of the four GFR-α (α1-4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.
Forme physique
Lyophilized from 10 mM Sodium Citrate + 50 mM NaCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Primary Postnatal Dorsal Root Ganglion Culture from Conventionally Slaughtered Calves.
Fadda A
PLoS ONE, 11(12), e0168228-e0168228 (2016)
Enrique Pimentel
Handbook of Growth Factors, Volume 2 null
Jacob L Roam et al.
Biomaterials, 35(24), 6473-6481 (2014-05-13)
Introduction of spatial patterning of proteins, while retaining activity and releasability, is critical for the field of regenerative medicine. Reversible binding to heparin, which many biological molecules exhibit, is one potential pathway to achieve this goal. We have covalently bound
Analysis of RET, ZEB2, EDN3 and GDNF genomic rearrangements in 80 patients with Hirschsprung disease (using multiplex ligation-dependent probe amplification).
Serra A
Annals of Human Genetics, 73(2), 147-151 (2009)
L F Lin et al.
Science (New York, N.Y.), 260(5111), 1130-1132 (1993-05-21)
A potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons was purified and cloned. Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. In embryonic
Protocoles
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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