SRP2020

TR, β 1 isoform human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonyme(s) : ERBA-beta, GRTH, NR1A2, THR1, THRB1
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.26

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥70% (SDS-PAGE)
• Forme: frozen liquid
• Poids mol.: ~53 kDa
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: 500 μg/mL
• Couleur: clear colorless
• Numéro d'accès NCBI: NM_000461
• Numéro d'accès UniProt: P10828
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... THRB(7068)

Description

Actions biochimiques/physiologiques

Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approximately 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approximately 250 amino acids. The amino-terminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TR α and TR β isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TR β gene generates two isoforms, TR β1 and TR β2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and transcription factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding protein–associated factor (PCAF), and CREB binding protein (CBP).

Forme physique

Clear and colorless frozen liquid solution

Notes préparatoires

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable