SRP0238

cMet Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• Synonyme(s) : HGFR, Hepatocyte growth factor receptor, RCCP2, Scatter factor receptor, met Proto-oncogene
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥60% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 74 kDa
• Conditionnement: pkg of 10 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: X54559
• Numéro d'accès UniProt: P08581
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... MET(4233)

Description

Description générale

Human cMet (GenBank Accession No. X54559), (amino acids 956-1390) with N-terminal GST tag, MW = 74 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 3 mM DTT and 20% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.