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SCJ4600029

Sigma-Aldrich

CF 594 hydrazide

suitable for fluorescence, ≥90% (HPLC), ≥90% (TLC)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Niveau de qualité

Pureté

≥90% (HPLC)
≥90% (TLC)

Forme

solid

Poids mol.

~743 g/mol

Solubilité

H2O: soluble

ε (coefficient d'extinction)

115000 at 593 nm

Rapport des absorbances

A280/593 nm 0.08

Fluorescence

λex 532 or 561 nm (Flow cytometry laser line)
λex 593 nm; λem 614 nm
λex 594 nm (Microscopy laser line)

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Description générale

CF594 is a deep red fluorescent dye spectrally similar to Alexa Fluor 594 and Texas Red® dye. When conjugated to proteins, CF594 is significantly brighter than Alexa Fluor 594 due to its high quantum yield and exceptional water solubility. CF594 also has excellent photostability, making it ideal for demanding applications such as confocal microscopy and single molecule imaging. These properties make CF594 the best deep-red dye for labeling proteins and nucleic acids. The dye is particularly useful in combination with our blue fluorescent CF350, green fluorescent CF488A and far red CF647 for multi-color imaging.

CF dye hydrazides can be used as a fixable polar tracers for visualizing cell morphology,or for gap junction studies. The dyes can be introduced into cells by microinjection. For conjugation to aldehyde or ketone groups (such as carbohydrate molecules after peroxidation with periodate) we recommend using the more reactive CF aminooxy dye forms.

Caractéristiques et avantages

1. Yields the brightest antibody conjugates among spectrally similar dyes. 2. Extremely photostable. 3. Highly water-soluble.

Notes préparatoires

Protect from light

Reconstitution

Dissolve in water

Informations légales

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.
Texas Red is a registered trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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