SAE0064

Angiotensin Converting Enzyme-2, ACE2

Human recombinant, ≥1000000 U/mg, expressed in HEK 293 cells

• Synonyme(s) : ACE-related carboxypeptidase, ACE2, Angiotensin-converting enzyme homolog (ACEH), COVID-19 receptor, Coronavirus receptor, Metalloprotease MPROT15
• Numéro de classification (Commission des enzymes): 3.4.17.23 (BRENDA, IUBMB)
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Niveau de qualité: 100; 200
• Produit recombinant: expressed in HEK 293 cells
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Activité spécifique: ≥1000000 U/mg
• Poids mol.: ~85.9 kDa by SDS-PAGE
• Impuretés: ≤1 EU/μg protein Endotoxin
• Numéro d'accès UniProt: Q9BYF1
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• Informations sur le gène: human ... ACE2(59272)

Description

Description générale

The gene for angiotensin converting enzyme-2 (ACE-2) is mapped to human chromosome Xp22.2. This protein is the first known human homologue of ACE. ACE2 has been identified from 5′ sequencing of a human heart failure ventricle cDNA library. It contains a signal peptide, one metalloprotease active site and a transmembrane domain. ACE-2 is a secreted and an integral membrane protein. It is expressed predominantly on the endothelium.

Application

Angiotensin Converting Enzyme-2 (ACE2) has been used in the ACE-2 inhibition assays for inhibitor selectivity studies. It has also been used for identifying potent ACE2-blocking monoclonal antibodies in an on-chip assay and in human-ACE2-blocking assay using a biolayer interferometry biosensor. This approach serves for rational vaccine designing and for the selection of robust immunotherapeutic agents against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

Actions biochimiques/physiologiques

Recombinant human Angiotensin Converting Enzyme-2 (ACE-2) is expressed in human HEK 293 cells as a C-terminally flag and histidine-tagged glycoprotein with a calculated molecular mass of 85.9 kDa (amino acids Gln18-Ser740, with a C-terminal 10-His tag). The DTT-reduced protein migrates as a 90-120 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein.

The angiotensin converting enzyme-2 (ACE-2) functions as carboxydipeptidase. The central role of ACE-2, is to counter ACE activity by reducing the bioavailability of angiotensin (Ang)-II and increasing the Ang(1-7) formation. ACE-2 is a part of the renin-angiotensin system (RAS). Many researches show that Ace-2 ensures the protection of peripheral tissues and might be efficient to treat RAS-related diseases. Also, an imbalance in Ace-2/Ang-(1-7) and ACE/Ang-II axes is important for the onset of cardiovascular diseases. Severe acute respiratory syndrome (SARS) and human coronavirus (HCoV)-NL63 infection viruses has been shown to use its surface protein spike (glycoprotein) to bind to human ACE-2 receptor. The S protein is cleaved into subunits, S1 and S2 during the COVID-19 infection. S1 contains the receptor binding domain (RBD) which enables coronaviruses to bind to the peptidase domain (PD) of ACE2. Thus, ACE2 has become a high focus research target for COVID-19 infection.

Définition de l'unité

One unit is defined as the amount of enzyme required to cleave 1 picomole of the fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH in one minute, in 37 °C, pH 7.5.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.