SAB4200719

Anti-Laminin antibody, Mouse monoclonal

clone LAM-89, purified from hybridoma cell culture

• Synonyme(s) : Anti-LAMA, Anti-LAMA1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: LAM-89, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: ~850 kDa
• Espèces réactives: feline, human, porcine
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Concentration: ~1.0 mg/mL
• Technique(s): ELISA: suitable; electron microscopy: suitable; immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane; immunofluorescence: suitable; immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections.
• Isotype: IgG1
• Numéro d'accès UniProt: P25391
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: cat ... Lama1(101083299) ; human ... LAMA1(284217) ; pig ... Lama1(100625589)

Description

Description générale

Monoclonal anti-laminin antibody (mouse IgG1 isotype) is derived from the LAM-89 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified laminin from human placenta. Monoclonal anti-laminin antibody specifically recognizes laminin from human, feline and porcine origin. The antibody shows reactivity with laminin and specifically staining basal membrane of blood vessels, epithelium, nerve and muscle fiber. Monoclonal anti-laminin shows no reaction with collagen IV, fibronectin, vitronectin and chondroitin sulfate type A, B or C. Laminins are complexed extracellular glycoproteins which assemble the basal components of the basement membrane (BM). Each laminin is a heterotrimer (~850kDa) comprising of an α (~400kDa), β (~200kDa) and γ (~200kDa) chains all held together by disulfide bonds.

Spécificité

Monoclonal Anti-Laminin antibody specifically recognizes Laminin from human, feline and porcine origin.

Immunogène

purified Laminin from human Placenta

Application

Monoclonal Anti-Laminin is recommended to use in various immunochemical assays, including immunohistochemistry, immunoblotting (~850 kDa), immunofluorescence, Enzyme linked immunosorbent assay (ELISA) and electron microscopy.

Actions biochimiques/physiologiques

Laminins contribute to the structure of the extracellular matrix (ECM). It also plays a major role in the regulation of variety of cellular processes, such as the rates of cell proliferation, differentiation, adhesion, migration, cell shaping, phenotype stability and resistance to apoptosis. Laminin polymers forms supramolecular network with collagen IV polymers through heparan sulfate proteoglycan and by nidogen (entactin) and plays an important role in the basement membrane (BM) stability. In mouse, mutations in the gene is associated with the development of muscular dystrophy.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable