SAB4200547

Anti-IDH1 (R132S) antibody, Mouse monoclonal

clone SMab-1, purified from hybridoma cell culture

• Synonyme(s) : Monoclonal Anti-IDCD, Monoclonal Anti-IDH, Monoclonal Anti-IDH1 (R132S) antibody produced in mouse, Monoclonal Anti-IDP, Monoclonal Anti-IDPC, Monoclonal Anti-NADP(+)-specific ICDH, Monoclonal Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Monoclonal Anti-Oxalosuccinate decarboxylase, Monoclonal Anti-PICD, Monoclonal Anti-isocitrate dehydrogenase 1 (NADP+), soluble, NADP-dependent isocitrate dehydrogenase, peroxisomal
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: SMab-1, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~43 kDa
• Espèces réactives: human
• Concentration: ~1 mg/mL
• Technique(s): immunoblotting: 0.25-0.5 μg/mL using using total cell extracts of HEK-293T cells overexpressing IDH1^R132S
• Numéro d'accès UniProt: O75874
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: mutation (Arg132Ser)
• Informations sur le gène: human ... IDH1(3417)

Description

Description générale

Monoclonal Anti-IDH1 (R132S) (mouse IgG1 isotype) is derived from the hybridoma SMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized by a peptide. Isocitrate dehydrogenase 1 (IDH1) is a homodimeric, nicotinamide adenine dinucleotide phosphate (NADP)-specific enzyme. It is localized in the peroxisomes and cytoplasm. IDH1 is mapped on the human chromosome at 2q34.

Spécificité

Monoclonal Anti- IDH1 (R132S) recognizes only human R132S mutated IDH1 and does not cross react with others.

Immunogène

immunized a peptide corresponding to the mutation R132S of human IDH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-IDH1 (R132S) antibody produced in mouse may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Actions biochimiques/physiologiques

Isocitrate dehydrogenase (IDH) plays an important role in catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate (αKG). Mutations in this gene are associated with several cancers, such as glioblastoma, acute myeloid leukemia (AML) and chondrosarcoma. Mutation in IDH1 specific to Arg¹³² results in the formation of 2-hydroxyglutarate (2HG) instead of αKG. 2HG hinders DNA and histone methylation resulting in alteration of gene transcription.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable