SAB4200082
Monoclonal Anti-Maltose Binding Protein (MBP) antibody produced in rat
1.0 mg/mL, clone MBP 7G4, purified immunoglobulin
Synonyme(s) :
Anti-MBP
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Source biologique
rat
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
MBP 7G4, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~42 kDa
Espèces réactives
wide range
Concentration
1.0 mg/mL
Technique(s)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using MBP recombinant protein
Isotype
IgG2a
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
MBP is part of a large class of proteins that aid in the uptake of small molecules. While it naturally resides in the periplasm, MBP can also be expressed at high yields in the cytoplasm. For different proteins, increased solubility, enhanced stability and markedly improved yields have been reported after fusion to MBP.
Monoclonal Anti-Maltose Binding Protein (MBP) (rat IgG2a isotype) is derived from the hybridoma MBP 7G4 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with MBP-fusion protein.1 The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. Monoclonal Anti- Maltose Binding Protein (MBP) is specific for MBP.
Spécificité
Monoclonal Anti- Maltose Binding Protein (MBP) is specific for MBP.
Immunogène
MBP-fusion protein
Application
Monoclonal Anti-Maltose Binding Protein (MBP) antibody produced in rat is suitable for:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
Monoclonal antibodies recognizing specifically MBP are useful in various immunotechniques for identifying the expression of an MBP fusion protein in bacteria or in cells transfected with MBP fusion protein expressing vectors.
Actions biochimiques/physiologiques
Maltose binding protein (MBP) tag is known to be used in genetic engineering to create a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest or with the biodistribution of the MBP tagged product. The expression of polypeptides in-frame with maltose binding protein (MBP) allows for their easy purification from bacterial extracts under mild conditions, which employ a single affinity chromatographic step on amylose resin. This system and others based on the expression of fusion proteins utilize a specific protease cleaving site to facilitate correct cleavage of the fusion protein. Thus, the MBP system incorporates a factor Xa cleavage site at the carboxy terminus of the MBP sequence, and cleavage by factor Xa separates MBP from its partner protein. Many recombinant proteins have been engineered with MBP tags to facilitate the detection, isolation and purification of the proteins.
Forme physique
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended use, freeze at -20 °Cin working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Outil de sélection de produits.
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Comparative analyses of ubiquitin-like ATG8 and cysteine protease ATG4 autophagy genes in the plant lineage and cross-kingdom processing of ATG8 by ATG4
Seo E, et al.
Autophagy, 12(11), 2054-2068 (2016)
Overview of affinity tags for protein purification
Kimple ME, et al.
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 73(1), 9-9 (2013)
José J Rodríguez-Herva et al.
Cellular microbiology, 14(5), 669-681 (2012-01-12)
The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in
A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses
Rodriguez HJ, et al.
Cellular Microbiology, 14(5), 669 ?681-669 ?681 (2016)
Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
Costa S, et al.
Frontiers in Microbiology, 5, 63-63 (2014)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique