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SAB4200031

Anti-TNPO2 (C-terminal) antibody produced in rabbit

Name: Anti-TNPO2 (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Importin 3, Anti-Karyopherin beta-2b, Anti-Transportin-2

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About This Item

Code UNSPSC :

12352203

Nomenclature NACRES :

NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~95 kDa

Espèces réactives

human

Conditionnement

antibody small pack of 25 μL

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over expressing human TNPO2
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human TNPO2
western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human TNPO2

Numéro d'accès UniProt

O14787

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TNPO2(30000)

Description générale

Transportin 2 (TNPO2 or TRN2) is a member of the karyopherin family of transport receptors. This protein exists in two isoforms TRN2a and TRN2b. The TNPO2 gene is located on human chromosome 19p13.2.

Spécificité

Anti-TNPO2 (C-terminal) recognizes human TNPO2 isoform b.

Application

Anti-TNPO2 (C-terminal) antibody produced in rabbit may be used in:

immunoblotting
immunoprecipitation
immunofluorescence

Actions biochimiques/physiologiques

Transportin 2 (TNPO2) is involved in a novel nuclear protein import and export pathway. This protein specifically binds to M9-containing proteins such as heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) as well as the nucleocytoplasmic shuttling sequence (HNS) region of HuR, but not to the classical nuclear localization signal (NLS)-containing proteins. TNPO2 protein interacts with a similar set of RNA-binding proteins in an RAs-related nuclear protein (Ran)- guanosine triphosphate (GTP) sensitive manner.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Transportin2 functions as importin and mediates nuclear import of HuR.

Stephan Güttinger et al.

Proceedings of the National Academy of Sciences of the United States of America, 101(9), 2918-2923 (2004-02-26)

The RanGTP-binding nuclear transport receptors transportin1 (TRN1) and transportin2 (TRN2) are highly similar in sequence but are reported to function in nuclear import and export, respectively. Here we show that TRN2 possesses properties of a nuclear import receptor. TRN1/2 both

Transportins 1 and 2 are redundant nuclear import factors for hnRNP A1 and HuR.

Ana Rebane et al.

RNA (New York, N.Y.), 10(4), 590-599 (2004-03-24)

Several mRNA-binding proteins, including hnRNP A1 and HuR, contain bidirectional transport signals that mediate both their nuclear import and export. Previously, Transportin 1 (Trn1) was identified as a mediator of hnRNP A1 import, whereas the closely related protein Transportin 2

Gene expression analysis reveals schizophrenia-associated dysregulation of immune pathways in peripheral blood mononuclear cells.

Erin J Gardiner et al.

Journal of psychiatric research, 47(4), 425-437 (2012-12-12)

Peripheral blood mononuclear cells (PBMCs) represent an accessible tissue source for gene expression profiling in schizophrenia that could provide insight into the molecular basis of the disorder. This study used the Illumina HT_12 microarray platform and quantitative real time PCR

IPO3-mediated Nonclassical Nuclear Import of NF-κB Essential Modulator (NEMO) Drives DNA Damage-dependent NF-κB Activation.

Byounghoon Hwang et al.

The Journal of biological chemistry, 290(29), 17967-17984 (2015-06-11)

Activation of IκB kinase (IKK) and NF-κB by genotoxic stresses modulates apoptotic responses and production of inflammatory mediators, thereby contributing to therapy resistance and premature aging. We previously reported that genotoxic agents induce nuclear localization of NF-κB essential modulator (NEMO)

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