S8409

Superoxide Dismutase from bovine liver

ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)

• Synonyme(s) : SOD, Superoxide: superoxide oxidoreductase
• Numéro CAS: 9054-89-1
• Numéro de classification (Commission des enzymes): 1.15.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-943-0
• Numéro MDL: MFCD00132404
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: ammonium sulfate suspension
• Activité spécifique: 2,000-6,000 units/mg protein (biuret)
• Poids mol.: 32.5 kDa
• Numéro d'accès UniProt: P00442
• Température de stockage: 2-8°C
• Informations sur le gène: cow ... SOD1(281495)

Description

Description générale

Research area: Cell Signaling

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Application

Superoxide Dismutase from bovine liver has been used:

• for measuring mitochondrial hydrogen peroxide production in skeletal muscle
• as a positive control in Cu(II) binding stability assay
• as a positive control in superoxide dismutase assay
• in a study to assess the inactivation of endothelial-derived relaxing factor by oxidized lipoproteins.
• in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

Actions biochimiques/physiologiques

Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Définition de l'unité

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Forme physique

Suspension in 3.8 M (NH₄)₂SO₄, pH 7.0

Remarque sur l'analyse

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)