S7199

Monoclonal Anti-SERCA3 antibody produced in mouse

~1.0 mg/mL, clone PL/IM430, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-ATP2A3, Anti-ATPase, Ca(2+)-Transporting, Ubiquitous, Anti-Sarcoplasmic reticulum Ca(2+)-ATPase 3
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: PL/IM430, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~100 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using platelet total cell extract.
• Isotype: IgG1
• Numéro d'accès UniProt: Q93084
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ATP2A3(489)

Description

Description générale

Monoclonal Anti-SERCA3 (mouse IgG1 isotype) is derived from the hybridoma PL/IM430 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The ATPase sarcoplasmatic/endoplasmatic reticulum Ca²⁺ transporting 3 (ATP2A3) gene codes for Ca²⁺-ATPase3 from the sarco/endoplasmic reticulum (SERCA3) and the coded protein is expressed in endothelial and epithelial tissues. The human SERCA3 gene gives rise to six 3¢-end splice variants encoding proteins varying in their C-terminal part and termed SERCA3a-f. SERCA3 gene is mapped to human chromosome 17p13.3.

Spécificité

Monoclonal Anti-SERCA3 recognizes human SERCA3.

Application

Monoclonal Anti-SERCA3 antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• western blotting
• immunoprecipitation
• immunohistochemistry

Actions biochimiques/physiologiques

Sarco/endoplasmic calcium ATPase pumps (SERCA) protein plays a pivotal role in intracellular calcium [Ca²⁺] homeostasis by lowering the cytoplasmic [Ca²⁺] concentration. Expression of sarco/endoplasmic reticulum (SERCA3) gene is reduced in gastric/colon carcinoma and leukemia cell types.The ATPase sarcoplasmatic/endoplasmatic reticulum Ca²⁺ transporting 3 (ATP2A3) gene may serve as an important target for salinomycin. Overexpression of this gene stimulates apoptosis. SERCA3 protein plays an important role in Ca²⁺ transport.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)