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RAB0490

Sigma-Aldrich

Human soluble TNF Receptor 2 ELISA Kit

for serum, plasma for serum, plasma, cell culture supernatant and urine

Synonyme(s) :

sTNFRII

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type plasma
sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

The Human sTNF RII ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human sTNF RII in serum, plasma, cell culture supernatants and urine.

Immunogène

Recombinant Human TNF RII

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Actions biochimiques/physiologiques

Tumor necrosis factor receptor 2 (TNFR2) plays a vital role in inducing signals for various cellular process, including proliferation of thymocytes and peripheral T cells, T cell secretion of granulocyte-macrophage colony-stimulating factor, inhibition of early hematopoiesis, and the inactivation and clearance of TNF by the kidney. Mutation in the gene leads to hematopoietic cell malignancies, neuroblastoma, glioma, cervical and ovarian carcinoma. TRAF2 interacts with TNFR2 and activates NF-kappa B (NF-κB) pathway. TNFR2, which lacks death domains, modulates the effect of other death receptor TNFR1, which induces T cell death. TNFR2 plays an essential role in TNF-α–induced leukocyte-endothelial interaction. TNFR2 is considered as a potential therapeutic alternative, as it has capacity to nullify the common side effects associated with drugs targeting TNF-α or p55.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The TNF and TNF receptor superfamilies: integrating mammalian biology.
Locksley RM, et al.
Cell, 104(4), 487-501 (2001)
Tumor necrosis factor alpha (TNF-alpha) receptor-II is required for TNF-alpha-induced leukocyte-endothelial interaction in vivo.
Chandrasekharan UM, et al.
Blood, 109(5), 1938-1944 (2007)
Human tumor necrosis factor receptor p75/80 (CD120b) gene structure and promoter characterization.
Santee SM, et al.
The Journal of Biological Chemistry, 271(35), 21151-21159 (1996)
TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40.
Rothe M, et al.
Science, 269(5229), 1424-1427 (1995)

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