Accéder au contenu
Merck
Toutes les photos(2)

Principaux documents

Voir toute la documentation

R5405

Sigma-Aldrich

Anti-Rabex-5 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-RAP1, Anti-Rab5 GDP/GTP exchange factor (RABGEF1), Anti-Rabaptin-5-associated exchange factor for Rab5

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~58 kDa

Espèces réactives

mouse, rat, human

Concentration

~1.0 mg/mL

Technique(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 2-5 μg/mL using whole extracts of mouse brain and human U87 cells

Numéro d'accès UniProt

Q9UJ41

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RABGEF1(27342)
mouse ... Rabgef1(56715)
rat ... Rabgef1(360797)

Description générale

Anti-Rabex-5 (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at N-terminal of human Rabex-5, with an added cysteine, conjugated to KLH. Rabex-5 contains multiple functional domains, including a vacuolar protein sorting-associated (VPS9) domain that possesses the GDP/GTP exchange factors (GEF) activity, a ubiquitin-binding A20-like zinc finger (ZnF) domain, and a coiled-coil domain.

Application

Anti-Rabex-5 (N-terminal) antibody may be used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

Rabex-5 forms a tight complex with Rabaptin-5 that is essential for endocytic membrane fusion. The bound Rabaptin-5 to Rabex-5 increases the GEF activity of Rabex-5 and fastens the generation of the active GTP-bound form of membrane associated Rab5.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 15(11), 1206-1218 (2014-08-02)
Listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on the activation of host cell RAB5 GTPases. Listeria monocytogenes must subsequently inhibit RAB5, however, in order to escape lysosome-mediated destruction. Relatively
Functional synergy between Rab5 effector Rabaptin-5 and exchange factor Rabex-5 when physically associated in a complex
Lippe R, et al.
Molecular Biology of the Cell, 12(7), 2219-2228 (2001)
The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain
Mattera R, et al.
Test, 281(10), 6874-6883 (2006)
The RAB5-GEF Function of RIN1 Regulates Multiple Steps During Listeria monocytogenes Infection.
Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 16(7), 796-796 (2015-06-09)
David Millrine et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(38), 10625-10630 (2016-09-08)
Immunomodulatory drugs (IMiDs) are a family of compounds derived from thalidomide. Binding of the IMiD molecule to the Lon protease Cereblon initiates the degradation of substrates via the ubiquitin proteasome pathway. Here, we show that Cereblon forms a complex with

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique