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R4001

Sigma-Aldrich

Ribonuclease A-agarose

ammonium sulfate suspension, 400-1,000 units/g agarose (One ml gel will yield 12-30 units)

Synonyme(s) :

Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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About This Item

Numéro de classification (Commission des enzymes):
Numéro MDL:
Code UNSPSC :
12352204

Source biologique

protein from bovine pancreas

Niveau de qualité

Matériaux

beaded agarose

Forme

ammonium sulfate suspension

Activité spécifique

400-1,000 units/g agarose (One ml gel will yield 12-30 units)

Poids mol.

~13,700

Température de stockage

2-8°C

Forme physique

Suspension in 2.0 M (NH4)2SO4 pH 7.0

Remarque sur l'analyse

Electrophoretically and enzymatically assayed. RNase A and B contents determined by SDS-PAGE. Protein determined by E1%/280.

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A spectrophotometric method for the measurement of ribonuclease activity.
M KUNITZ
The Journal of biological chemistry, 164(2), 563-568 (1946-08-01)
Marka van Blitterswijk et al.
PloS one, 7(11), e48983-e48983 (2012-11-17)
Progressive muscular atrophy (PMA) and amyotrophic lateral sclerosis (ALS) are devastating motor neuron diseases (MNDs), which result in muscle weakness and/or spasticity. We compared mutation frequencies in genes known to be associated with MNDs between patients with apparently sporadic PMA
Yuen Lai Shek et al.
Biochemistry, 52(4), 672-680 (2013-01-09)
We report the first application of volume and compressibility measurements to characterization of interactions between cosolvents (osmolytes) and globular proteins. Specifically, we measure the partial molar volumes and adiabatic compressibilities of cytochrome c, ribonuclease A, lysozyme, and ovalbumin in aqueous
Chunju Li et al.
Journal of biotechnology, 162(2-3), 283-288 (2012-09-25)
Ribonuclease A (RNase A) is a therapeutic enzyme with cytotoxic action against tumor cells. Its clinical application is limited by the short half-life and insufficient stability. Conjugation of albumin can overcome the limitation, whereas dramatically decrease the enzymatic activity of
Shalini Iyer et al.
The FEBS journal, 280(1), 302-318 (2012-11-23)
Angiogenin (Ang) is a potent inducer of neovascularization. Point mutations in human Ang have been linked to cancer progression and two neurodegenerative diseases: amyotrophic lateral sclerosis and Parkinson's disease. Intensive structural and functional analyses of Ang have been paramount in

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