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R3400

Sigma-Aldrich

Monoclonal Anti-c-K-Ras antibody produced in mouse

clone 234-4.2, purified immunoglobulin, buffered aqueous solution

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About This Item

Numéro MDL:
MFCD00239650
Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

234-4.2, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 21 kDa

Espèces réactives

mouse, human, rat

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10 μg/mL using normal skin tissue
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2κ

Numéro d'accès UniProt

P01116

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KRAS(3845)
mouse ... Kras(16653)
rat ... Kras(24525)

Description générale

c-K-Ras or KRAS (kirsten rat sarcoma-2 virus oncogene) is a small GTP-binding protein. It is located on human chromosome 12p12.1. KRAS has two splice variants, such as KRASA and KRASB (KRAS proto-oncogenes). Expression of KRASA is restricted to specific tissues whereas KRASB is expressed everywhere .

Spécificité

The antibody reacts with c-K-Ras and v-K-Ras p21. It crossreacts with cH-Ras and cN-Ras p21 on immunoblots.

Immunogène

recombinant p21 protein

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

KRAS (kirsten rat sarcoma-2 virus oncogene) mutation leads to poor prognosis of early rectal cancer.

Forme physique

Solution in 0.05 M sodium phosphate buffer, pH7.5, containing 0.2% gelatin and < 0.1% sodium azide

Notes préparatoires

Purified using protein A or G

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Jasminka Omerovic et al.
The Biochemical journal, 426(1), 65-72 (2009-11-20)
Oncogenic Ras mutations render the protein constitutively active and promote tumorigenesis via chronic stimulation of effector pathways. In A549 lung adenocarcinoma approx. 50% of the total Ras population is constitutively active, yet these cells display only weak activation of the
David A Iglesias et al.
Molecular cancer therapeutics, 12(12), 2847-2856 (2013-10-01)
Metformin is an oral biguanide commonly used for the treatment of type II diabetes and has recently been demonstrated to possess antiproliferative properties that can be exploited for the prevention and treatment of a variety of cancers. The mechanisms underlying
Candida Zuchegna et al.
Redox report : communications in free radical research, 27(1), 150-157 (2022-07-14)
Although the protooncogenes small GTPases Ras are redox-sensitive proteins, how they are regulated by redox signaling in the central nervous system (CNS) is still poorly understood. Alteration in redox-sensitive targets by redox signaling may have myriad effects on Ras stability
Harrison J Hocker et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(25), 10201-10206 (2013-06-06)
Aberrant signaling by oncogenic mutant rat sarcoma (Ras) proteins occurs in ∼15% of all human tumors, yet direct inhibition of Ras by small molecules has remained elusive. Recently, several small-molecule ligands have been discovered that directly bind Ras and inhibit
Kwang-jin Cho et al.
The Journal of biological chemistry, 287(52), 43573-43584 (2012-11-06)
Oncogenic mutant Ras is frequently expressed in human cancers, but no anti-Ras drugs have been developed. Since membrane association is essential for Ras biological activity, we developed a high content assay for inhibitors of Ras plasma membrane localization. We discovered
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