P7493

Anti-PCAF antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-p300/CBP associated factor
• Numéro MDL: MFCD03791929
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~88 kDa
• Espèces réactives: canine, human
• Technique(s): immunoprecipitation (IP): 5-10 μg using RIPA lysate with ~4x10⁷ human acute T cell leukemia Jurkat cells; microarray: suitable; western blot (chemiluminescent): 1:500 using whole extracts of dog MDCK kidney cells
• Numéro d'accès UniProt: Q92831
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KAT2B(8850)

Description

Description générale

PCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily.

Spécificité

Anti-PCAF recognizes human and dog PCAF. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.

Immunogène

This sequence is identical to mouse PCAF and varies by 2 amino acids residues in chicken.

synthetic peptide corresponding to the C-terminal (amino acids 817-832) of human PCAF with N-terminal added cysteine, conjugated to KLH.

Application

Anti-PCAF antibody is also suitable for use microarray, immunoblot, chemiluminescent western blot (1:500 using whole extracts of dog MDCK kidney cells), and immunoprecipitation (5-10 μg using RIPA lysate with approx. 4x10,000,000 human acute T cell leukemia Jurkat cells).

Anti-PCAF antibody produced in rabbit has been used in western blot and immunoprecipitation.

Actions biochimiques/physiologiques

PACF (p300/CBP association factor) functions as a transcriptional coactivator in association with p300 and CBP (CREB-binding protein). PCAF share high degree of sequence similarity with human and mouse GCN5 HAT (Histone acetyltransferases). PACF mediates the acetylation of core histone. It is also known for the acetylation of non-histone proteins such as p53, MyoD, TFIIE-b, TFIIE, GATA-1, EKLF, HMGI (Y), and several other cellular and viral proteins. PCAF possesses chromatin modification activity and modulation of transcription factors for the activation of transcription. It also promotes cell growth and development as myogenesis and also contribute to receptor-mediated cell signaling or activation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable