N6539

Anti-NUMB (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonyme(s) : Anti-S171
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Poids mol.: antigen ~70 kDa
• Espèces réactives: human, rat
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~1.0 mg/mL
• Technique(s): western blot: 1-2 μg/mL using rat brain extract (S1 fraction) and A431 cell lysate
• Numéro d'accès UniProt: P49757
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NUMB(8650) ; mouse ... Numb(18222) ; rat ... Numb(29419)

Description

Description générale

Numb gene with 13 exons is mapped to human chromosome 14q24.3. The protein comprises 651 amino acids. It belongs to the clathrin-associated sorting protein family with two domains namely phosphotyrosine-binding (PTB) and proline-rich region (PRR) domain. Mammalian Numb gene gives rise to at least four alternatively spliced transcripts that produce four protein isoforms Numb1-4, ranging from 65 to 72 kDa.

Numb homolog-NUMB is an adaptor protein and is aberrantly expressed in cancers.

Application

Anti-NUMB (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Actions biochimiques/physiologiques

Numb homolog-NUMB may act as a tumor suppressor. It also takes part in determining the cell fate. NUMB is involved in the endocytosis and trafficking of receptors.

Numb is a critical Notch antagonist and a conserved adapter protein that plays a role in the self-renewal of NSCs and neural differentiation in the central nervous system (CNS). In neural progenitors of CNS, the functional loss of numb is implicated in embryonic lethality accompanied by precocious neuronal differentiation. It has been suggested that a switch in numb isoform expression is a critical step in cortical development. Numb1 or numb3 isoforms that contain an insertion in the proline-rich region (PRRL), promote the proliferation of neural crest stem cells (NCSCs). Numb2 or numb4 isoforms without PRR insertions (PRRS) show ectopic expression and promote neuronal differentiation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)