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N6255

Sigma-Aldrich

β-Nicotinamide adenine dinucleotide phosphate–Agarose

saline suspension

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About This Item

Numéro MDL:
MFCD00171240
Code UNSPSC :
23151817

Forme

saline suspension

Ampleur du marquage

2-4 μmol per mL

Matrice

Cross-linked 4% beaded agarose

Activation de la matrice

cyanogen bromide

Fixation de matrice

ribose hydroxyls

Espaceur de matrice

11 atoms (adipic acid dihydrazide)

Température de stockage

2-8°C

Application

NADP-agarose is used for protein chromatography, affinity chromatography and nucleotide/coenzyme resins. NADP-agarose has been used to evaluate macroporous scaffolds for bone tissue engineering for biomedical applications.

Forme physique

Suspension in 0.5 M NaCl containing 0.02% thimerosal

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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D Wyrambik et al.
European journal of biochemistry, 97(2), 503-509 (1979-07-01)
Isoenzyme 2 of cinnamyl-alcohol dehydrogenase from soybean suspension cultures was purified about 3800-fold to apparent homogeneity by an improved purification procedure involving biospecific elution of the enzyme from a NADP+-agarose column. On sodium dodecylsulfate gels the dehydrogenase showed only one
J A Puértolas et al.
Acta biomaterialia, 7(2), 841-847 (2010-08-17)
There is an acknowledged need for shaping 3-D scaffolds with adequate porosity and mechanical properties for biomedical applications. The mechanical properties under static and cyclic compressive testing of dense and designed porous architecture bioceramic scaffolds based on the biphasic calcium
Sreelatha T Reddy et al.
Protein expression and purification, 26(2), 290-300 (2002-10-31)
Mannose 6-phosphate receptors (MPRs) form essential components of the lysosomal enzyme targeting system by binding newly synthesized acid hydrolases with high (nM) affinity. We report the use of Pichia pastoris as a host to efficiently express the extracytoplasmic ligand-binding domain
M R Prasad et al.
Archives of biochemistry and biophysics, 237(2), 535-544 (1985-03-01)
The present study describes the solubilization and purification of a NADPH-specific trans-2-enoyl-CoA reductase from rat liver microsomes. The final preparation was purified to near homogeneity and had a minimal molecular weight of 51,000 +/- 2,000, as judged by sodium dodecylsulfate
C Andriamampandry et al.
The Biochemical journal, 334 ( Pt 1), 43-50 (1998-08-07)
The gamma-hydroxybutyrate biosynthetic enzyme succinic semialdehyde reductase (SSR) was purified to homogeneity from rat brain. Peptides were generated by tryptic cleavage and sequenced. PCR primers were designed from the amino acid sequences of two of the peptides showing a similarity
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