MTOX1093

MRP3 Knockout Caco-2 Cells

human male colorectal tissue (Source Disease: colon adenocarcinoma)

• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• product name: MRP3 Knockout Caco-2 Cells, one vial
• Source biologique: human male colorectal tissue (Source Disease: colon adenocarcinoma)
• Forme: liquid
• Technique(s): drug transporter assay: suitable; permeability assay: suitable
• Application(s): ADME/TOX
• Température de stockage: −196°C
• Informations sur le gène: human ... ABCC3(8714)

Description

Description générale

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP3 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC3 (MRP3) efflux transporter.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Caractéristiques et avantages

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional knockout of the MRP3 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP3 substrate
• The vial format enables the MRP3 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Informations légales

ADME/Tox Cell Lines License

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable