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MSP03

Sigma-Aldrich

Membrane Scaffold Protein 1D1(-)

recombinant, expressed in E. coli, buffered aqueous solution

Synonyme(s) :

Membrane Scaffold Protein

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.26

Produit recombinant

expressed in E. coli

Pureté

≥90% (SDS-GE)

Forme

buffered aqueous solution

Poids mol.

22,043.9 Da

Solubilité

water: soluble

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

Research area: Cell structure. Membrane Scaffold Protein 1D1 (MSP1D1) is a variant of MSP1 where the first eleven amino acids in the helix 1 portion are deleted of the original MSP1 sequence. It consists of an N-terminal histidine-tagged protein with a tobacco etch virus (TEV) protease cleavage site that is present between the protein sequence and histidine-tag. MSP1D1(–)variant of MSP1D1 lacks the histidine-tag and the TEV protease-recognition sequence.

Application

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Actions biochimiques/physiologiques

Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable. The concept of this technology is derived from high-densitylipoprotein (HDL) particles and apolipoprotein, their primary proteincomponent. The nano-disc is a non-covalent structure consisting of MSP that isgenetically engineered to mimic the activity of apolipoprotein A1 (Apo-A1) andphospholipid bilayer. In the presence of synthetic phospholipids, MSP1D1 canself-assemble into discoidal nanoparticlesknown as nano-discs. These nano-discs are suitable for studying membraneproteins and are applicable in the field of medicine and biotechnology.

Informations légales

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
  • 7,691,414 Membrane scaffold proteins
  • 7,662,410 Membrane scaffold proteins and embedded membrane proteins
  • 7,622,437 Tissue factor compositions and methods
  • 7,592,008 Membrane scaffold proteins
  • 7,575,763 Membrane scaffold proteins and tethered membrane proteins
  • 7,083,958 Membrane scaffold proteins
  • 7,048,949 Membrane scaffold proteins

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Membrane Scaffold Protein 1E3D1 recombinant, expressed in E. coli, buffered aqueous solution

Sigma-Aldrich

MSP08

Membrane Scaffold Protein 1E3D1

TEV protéase

Sigma-Aldrich

T4455

TEV protéase

Ramona Faas et al.
Biotechnology reports (Amsterdam, Netherlands), 17, 45-48 (2018-01-31)
Membrane scaffold proteins (MSPs) are synthetic derivatives of apolipoprotein A-I, a major protein component of human high-density lipoprotein complexes. The most common among these is the variant MSP1D1, which has been in the focus of research on membrane mimetics in

Protocoles

Membrane Scaffold Proteins: Nanodisc Formation

Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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