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MBD0010

Sigma-Aldrich

Inactivated Salmonella enterica

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.51

Niveau de qualité

200

Forme

liquid

Concentration

>10^8 bacteria/ml

Technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Inactivated Salmonella enterica can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Salmonella enterica serotype Typhimurium (also S. Typhimurium) is a rod-shaped gram-negative bacterium of the family Enterobacteriaceae. It is an important food-borne pathogen that in humans causes intestinal inflammation, diarrhea, and the presence of neutrophils in stool samples.2 Growth of S. enterica in the gut lumen causes depletion of the healthy microbial community.3 Infection with Salmonella can induce inflammation and increase the risk for inflammatory bowel disease (IBD)4

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Inactivated bacteria are provided at >10^8 bacteria/ml concentration in TE buffer pH 8.0.It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Caractéristiques et avantages

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Forme physique

Liquid -The inactivated bacteria is provided at >10^8 bacteria/ml in TE buffer pH 8.0

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Inna Sekirov et al.
Gut microbes, 1(1), 30-41 (2011-02-18)
Gastrointestinal infections involve an interactive tripartite relationship between the invading pathogen, the host, and the host's resident intestinal microbiota. To characterize the host inflammatory response and microbiota alterations during enteric salmonellosis, C57BL/6 mice were pre-treated with a low dose of
Kim O Gradel et al.
Gastroenterology, 137(2), 495-501 (2009-04-14)
Various commensal enteric and potentially pathogenic bacteria may be involved in the pathogenesis of inflammatory bowel diseases (IBD). We compared the risk of IBD between a cohort of patients with documented Salmonella or Campylobacter gastroenteritis and an age- and gender-matched
Fecal leukocytes in diarrheal illness.
J C Harris et al.
Annals of internal medicine, 76(5), 697-703 (1972-05-01)

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