MAK165
Fluorimetric Hydrogen Peroxide Assay Kit
sufficient for 500 fluorometric tests (red fluorescence)
Synonyme(s) :
Hydrogen Peroxide Quantification Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 500 fluorometric tests (red fluorescence)
Méthode de détection
fluorometric
Maladie(s) pertinente(s)
cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders
Température de stockage
−20°C
Catégories apparentées
Description générale
Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.
Principe
The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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