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MAK091

Sigma-Aldrich

Hexokinase Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

Hexokinase Detection Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cancer; neurological disorders; hematological disorder

Température de stockage

−20°C

Informations sur le gène

human ... GCK(2645) , HK1(3098) , HK2(3099) , HK3(3101)
mouse ... GCK(103988) , HK1(15275) , HK2(15277) , HK3(212032)
rat ... GCK(24385) , HK1(25058) , HK2(25059) , HK3(25060)

Description générale

Hexokinases, which catalyze the ATP-dependent phosphorylation of aldo- and keto-hexoses to hexose-6-phosphate, catalyze the first step in a number of important biochemical pathways. In mammalian tissues, glucose is the predominant substrate for the hexokinases. There are four isozymes of hexokinase in mammalian tissue, (HK-I, -II, -III, and -IV), which differ in their kinetic and regulatory properties as well as tissue distribution and cofactor use. The hexokinases catalyze the first step in most of the relevant glucose metabolism pathways. Alterations in hexokinase activity are associated with multiple disorders such as X-linked muscular dystrophy, hemolytic anermias, and cancer.

Application

Hexokinase Colorimetric Assay Kit has been used to measure the activity of hexokinase.

Adéquation

Suitable for the measurement of Hexokinase activity, analysis of glucose metabolism and cell signaling, and screening anti-diabetic drugs in serum, animal tissues (liver, heart, kidney etc.), and cell culture (adherent or suspension cell).

Principe

The Hexokinase Colorimetric Assay kit provides a simple and direct procedure for measuring hexokinase activity in a variety of samples. Hexokinase activity is determined by a coupled enzyme assay, in which glucose is converted to glucose-6-phosphate by hexokinase, which is oxidized by glucose-6-phosphate dehydrogenase to form NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the hexokinase activity present. One unit of HK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition.
Vivek P, et al.
Indian J. Agri. Sci., 88(2), 222-228 (2018)
Md Wasim Khan et al.
Biochimica et biophysica acta. Molecular basis of disease, 1865(3), 678-687 (2018-12-14)
Hexokinase domain containing 1, a recently discovered putative fifth hexokinase, is hypothesized to play key roles in glucose metabolism. Specifically, during pregnancy in a recent genome wide association study (GWAS), a strong correlation between HKDC1 and 2-h plasma glucose in
Samuel E Schriner et al.
Journal of medicinal food, 19(3), 318-323 (2016-03-18)
The root and rhizome extract of Rhodiola rosea has been extensively used in traditional medicine to improve physical and mental performance and to protect against stress. We, and others, have reported that R. rosea can extend lifespan in flies, worms
Marybeth Anderson et al.
Oncotarget, 8(34), 56081-56094 (2016-06-01)
Pancreatic ductal adenocarcinoma (PDAC) is a KRAS-driven cancer with a high incidence of metastasis and an overall poor prognosis. Previous work in a genetically engineered mouse model of PDAC showed glucose metabolism to be important for maintaining tumor growth. Multiple
Crystal structures of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii.
Nishimasu H, et al.
The Journal of Biological Chemistry, 282(13), 9923-9931 (2007)
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Protocoles

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

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