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Merck
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Principaux documents

MAK056

Sigma-Aldrich

Choline/Acetylcholine Quantification Kit

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

Synonyme(s) :

Choline/Acetylcholine Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.25

Utilisation

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

Méthode de détection

colorimetric
fluorometric

Température de stockage

−20°C

Description générale

Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.

Application

Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.

Adéquation

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principe

In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.

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Réf. du produit
Description
Tarif

Pictogrammes

Health hazardExclamation mark

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup


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Consulter la Bibliothèque de documents

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Elin Rebecka Carlsson et al.
Lipids in health and disease, 18(1), 169-169 (2019-09-07)
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Compromised epidermal barrier stimulates Harderian gland activity and hypertrophy in ACBP-/-mice
Bek S, et al.
Journal of Lipid Research, jlr-M060780 (2015)
Effects of Hibernation on the Enteric Nervous System of the Thirteen-lined Ground Squirrels.
Eliades L, et al.
Journal of Undergraduate Research, 1-6 (2014)
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Journal of inherited metabolic disease, 45(2), 278-291 (2021-12-23)
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