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MAK054

Sigma-Aldrich

α-Ketoglutarate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

cancer

Température de stockage

−20°C

Description générale

α-Ketoglutarate (α-KG) is a key intermediate in the Tricarboxylic Acid (TCA) Cycle, formed by the oxidative decarboylation of isocitrate by isocitrate dehydrogenase. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. α-KG is an important nitrogen transporter. This α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples.

Application

α-Ketoglutarate Assay Kit has been used to determine the activity of α-ketoglutarate.

Adéquation

Suitable for the measurement of a-Ketoglutarate in a variety of biological samples including cell and tissue lysates

Principe

α-KG concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the α-KG present. Typical sensitivities of detection for this kit are 0.01 to 10 nmoles.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Phoebe J Benson et al.
Frontiers in microbiology, 7, 475-475 (2016-04-20)
Interest in the production of carbon commodities from photosynthetically fixed CO2 has focused attention on cyanobacteria as a target for metabolic engineering and pathway investigation. We investigated the redirection of carbon flux in the model cyanobacterial species, Synechococcus elongatus PCC
Gunars Osis et al.
Physiological reports, 4(8) (2016-04-28)
The bicarbonate transporter, NBCe1 (SLC4A4), is necessary for at least two components of the proximal tubule contribution to acid-base homeostasis, filtered bicarbonate reabsorption, and ammonia metabolism. This study's purpose was to determine NBCe1's role in a third component of acid-base
Glutamine transporters are targets of multiple oncogenic signaling pathways in prostate cancer
White M A, et al.
Molecular Cancer Research, 480-480 (2017)
Shota Kawakami et al.
Oncology letters, 20(6), 351-351 (2020-10-31)
Isocitrate dehydrogenase 1 (IDH1) mutations are common in gliomas, acute myeloid leukemia, and chondrosarcoma. The mutation 'hotspot' is a single arginine residue, R132. The R132H mutant of IDH1 produces the 2-hydroxyglutarate (2-HG) carcinogen from α-ketoglutarate (α-KG). The reduction of α-KG
Shaohui Chen et al.
Stem cell research, 49, 102097-102097 (2020-12-04)
The developmental plasticity of embryonic stem cells (ESCs) is mainly controlled by well-characterized transcription factors, but additional factors, especially those related to metabolism that modulate this intrinsic program remain elusive. Here, using whole transcriptome analysis, we identified branched-chain amino acid
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Protocoles

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We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

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