M9532
Mannitol Dehydrogenase from Leuconostoc mesenteroides
lyophilized powder, ≥60 units/mg protein
Synonyme(s) :
polyol dehydrogenases, Mannitol:NAD oxidoreductase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Forme
lyophilized powder
Niveau de qualité
Activité spécifique
≥60 units/mg protein
Poids mol.
136 kDa
Température de stockage
−20°C
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Description générale
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
The molecular weight of mannitol dehydrogenase is 136 kDa.
Application
Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
This preparation is useful in the determination of mannitol in urine.
Actions biochimiques/physiologiques
Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.
Définition de l'unité
One unit will reduce 1.0 μmole of D-fructose per min in the presence of NADH at pH 5.3 at 30°C. One unit of enzyme, as defined above, is equivalent to approximately 1.8 units of enzyme, as described by Lunn et al., one unit will oxidize 1.0 μmole of D-mannitol per min in the presence of NAD at pH 8.6 at 40°C.
Forme physique
Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol
Notes préparatoires
Partially purified
Remarque sur l'analyse
Assayed according to the method of Yamanaka, Meth. Enzymol., 41(B), 138 (1975).
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
Certificats d'analyse (COA)
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Enzymatic production of D-mannitol with the Leuconostoc pseudomesenteroides mannitol dehydrogenase coupled to a coenzyme regeneration system
Parmentier S
Biocatalysis and Biotransformation, 23, 1-7 (2005)
Polyol metabolism in homopterans at high temperatures: accumulation of mannitol in aphids (Aphididae: Homoptera) and sorbitol in whiteflies (Aleyrodidae: Homoptera)
Hendrix D.L. , Salvucci, M.E.
Comparative Biochemistry and Physiology, 120, 487-494 (1998)
J Aarnikunnas et al.
Applied microbiology and biotechnology, 59(6), 665-671 (2002-09-13)
The N-terminal amino acid sequences of the intact protein and three tryptic peptides from a 41 kDa protein purified from a commercial mannitol dehydrogenase (MDH) enzyme preparation of Leuconostoc mesenteroides ATCC-9135 were determined. Oligonucleotides deduced from these peptide sequences were
Kevin Blackburn et al.
Rapid communications in mass spectrometry : RCM, 24(7), 1009-1016 (2010-03-10)
Plant cells secrete a wide variety of defense-related proteins into the extracellular space or apoplast in response to pathogen attack. One of these, mannitol dehydrogenase (MTD), is normally a cytoplasmic enzyme whose primary role is the regulation of intracellular levels
P G Lunn et al.
Clinica chimica acta; international journal of clinical chemistry, 183(2), 163-170 (1989-08-15)
A need for a simple method for the determination of mannitol in urine has arisen because of the use of this monosaccharide in intestinal permeability tests. A rapid spectrophotometric assay for mannitol is presented based on the use of the
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