L9639

Ricin B chain from Ricinus communis (castor bean)

buffered aqueous solution

• Numéro CAS: 96638-29-8
• Numéro MDL: MFCD00166673
• Code UNSPSC : 12352200

Propriétés

• Forme: buffered aqueous solution
• Température de stockage: 2-8°C

Description

Actions biochimiques/physiologiques

Ricin B chain has an affinity for galactose and saccharides containing nonreducing terminal β-galactosyl units. This affinity allows the B chain to bind to cell surface glycoconjugates. In the intact ricin toxin, the surface binding by the B chain allows the A chain to enter into the cell. The saccharide binding capability of ricin B chain is independent of the presence of the A chain.

Autres remarques

Ricinus communis toxin (also named RCA₆₀, RCA_II, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.

Qualité

Electrophoretically pure (SDS-PAGE)

Forme physique

Solution in 10 mM phosphate, pH 6.5, containing 0.15 M NaCl, 10 mM galactose, 0.5 mM dithioerythritol and 0.02% sodium azide

Notes préparatoires

Highly purified by affinity chromatography

Remarque sur l'analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable