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L3287

Sigma-Aldrich

Escort™ IV Transfection Reagent

Lipid reagent for transient and stable transfection of mammalian and insect cells.

Synonyme(s) :

Gene delivery

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About This Item

Code UNSPSC :
41106502
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

100

Forme

liquid (aqueous solution)

Utilisation

1 mL sufficient for 160-500 transfections

Concentration

1 mg/mL

Technique(s)

transfection: suitable

Température de stockage

2-8°C

Catégories apparentées

Description générale

Escort™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort™ IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort™ IV, see the Transfection Reagent Selection Guide.

Caractéristiques et avantages

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of cell lines
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for Sf9, Sf21 and S2 insect cells

Composants

Escort™ IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort™ IV is confidential.

Attention

Do not freeze.

Principe

A stable complex is formed when Escort™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Informations légales

Escort is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Zev Bryant et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(3), 772-777 (2006-12-22)
Myosin VI supports movement toward the (-) end of actin filaments, despite sharing extensive sequence and structural homology with (+)-end-directed myosins. A class-specific stretch of amino acids inserted between the converter domain and the lever arm was proposed to provide
Manveen K Gupta et al.
Antioxidants & redox signaling, 8(5-6), 1081-1093 (2006-06-15)
Cardiac myocytes, upon exposure to increasing doses of norepinephrine (NE), transit from hypertrophic to apoptotic phenotype. Since reactive oxygen species (ROS) generation is attributed to both phenomena, the authors tested whether an elevation in intracellular ROS level causes such transition.
Melissa D Jordan et al.
Chemical senses, 34(5), 383-394 (2009-03-19)
Moths recognize a wide range of volatile compounds, which they use to locate mates, food sources, and oviposition sites. These compounds are recognized by odorant receptors (OR) located within the dendritic membrane of sensory neurons that extend into the lymph
R'ada Massarwa et al.
Developmental cell, 12(4), 557-569 (2007-04-11)
Formation of syncytial muscle fibers involves repeated rounds of cell fusion between growing myotubes and neighboring myoblasts. We have established that Wsp, the Drosophila homolog of the WASp family of microfilament nucleation-promoting factors, is an essential facilitator of myoblast fusion
Victoria J Hammond et al.
The Analyst, 133(1), 71-75 (2007-12-19)
Sol-gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitro intracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol-gel matrix was selected for
Afficher tous les articles scientifiques apparentés

Articles

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocoles

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Contenu apparenté

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

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