L0520

Lactoferrin from human milk

≥85% (SDS-PAGE), lyophilized powder

• Synonyme(s) : Growth-inhibiting protein 12, Lactotransferrin, Talalactoferrin
• Numéro CAS: 936541-36-5
• Numéro MDL: MFCD00164307
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.61

Propriétés

• Source biologique: human milk
• Pureté: ≥85% (SDS-PAGE)
• Forme: lyophilized powder
• Poids mol.: 82.4 kDa
• Composition: Protein, ≥90% biuret
• Technique(s): microbiological culture: suitable
• Solubilité: phosphate buffer: 1 mg/mL, clear to slightly hazy (0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C)
• Numéro d'accès UniProt: P02788
• Température de stockage: 2-8°C
• InChI: 1S/C35H53N9O12/c1-16(2)27(43-33(53)23(14-26(47)48)41-25(46)15-38-29(49)18(5)39-31(51)21(36)13-24(37)45)34(54)40-19(6)30(50)42-22(12-20-10-8-7-9-11-20)32(52)44-28(17(3)4)35(55)56/h7-11,16-19,21-23,27-28H,12-15,36H2,1-6H3,(H2,37,45)(H,38,49)(H,39,51)(H,40,54)(H,41,46)(H,42,50)(H,43,53)(H,44,52)(H,47,48)(H,55,56)/t18-,19-,21-,22-,23-,27-,28-/m0/s1
• Clé InChI: QCBUWCQOKPLTDZ-PKRULZLPSA-N
• Informations sur le gène: human ... LTF(4057)

Description

Application

Lactoferrin was used to grow Streptococcus mutans in an iron-limiting medium. It was used to test if lactoferrin impedes epithelial cell adhesion in vitro.

Actions biochimiques/physiologiques

Lactoferrin is an iron binding protein. It is structurally similar to transferrin, the plasma iron transport protein; but lactoferrin has a much higher affinity for iron (250 fold). It is very abundant in colostrum and small amounts can also be found in tears, saliva, mucous secretions and in the secondary granules of neutrophils. It is made by mucosal epithelium and neutrophils and is released by these cells in response to inflammatory stimuli. Bacterial growth is inhibited by its ability to sequester iron and also permeabilize bacterial cell walls by binding to lipopolysaccharides through its N-terminus. Lactoferrin can inhibit viral infection by binding tightly to the viral envelope protein. This prevents cell-virus fusion by blocking the binding domain. Lactoferrin appears to activate host defense systems in part by stimulating the release of interleukin-8, a neutrophil activator. It may also be involved in antibody and interleukin synthesis, lymphocyte proliferation and complement activation.

Qualité

Contains iron, see certificate of analysis for lot specific information.

Notes préparatoires

Chromatographically purified.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable