I8638
Anti-Integrin β1 antibody, Mouse monoclonal
clone W1B10, purified from hybridoma cell culture
Synonyme(s) :
Anti-CD29
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified from hybridoma cell culture
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
W1B10, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen 100-140 kDa
Contient
15 mM sodium azide
Espèces réactives
chicken
Technique(s)
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
chicken ... ITGB1(374058)
Description générale
Monoclonal anti-β-1-Chicken Integrin (mouse IgG1 isotype) is derived from the W1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken integrin from fibroblasts of 11 days old chicken embryos. Chicken integrin is a heterodimer consisting of the common β-1 subunit and two α subunits (α-5 or α-3). The β-1 subunit of chicken integrin is a 803-amino acid transmembrane glycoprotein consisting of a large extracellular domain and a short cytoplasmic domain.
Spécificité
The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β1 class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β1 subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.
Immunogène
chicken integrin.1
Application
Anti-Integrin β1 antibody, Mouse monoclonal has been used in western blot, fluorescent activated cell sorter (FACS) analysis.
Actions biochimiques/physiologiques
Integrin binding to extracellular matrix (ECM) ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. The monoclonal anti-b-1 chicken integrin antibody (clone W1B10) has been used to inhibit chicken muscle cell adhesion in cell cultures.
Forme physique
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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beta1-Integrin-collagen interaction reduces chondrocyte apoptosis
Cao L, et al.
Matrix Biology, 18(4), 343-355 (1999)
Bonnie M Marsick et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(1), 282-296 (2012-01-06)
The development of a functioning neural network relies on responses of axonal growth cones to molecular guidance cues that are encountered en route to their target tissue. Nerve growth factor (NGF) and neurotrophin-3 serve as attractive cues for chick embryo
Andrea Ketschek et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(36), 12185-12197 (2010-09-10)
The initiation of axonal filopodia is the first step in the formation of collateral branches and synaptic structures. In sensory neurons, nerve growth factor (NGF) promotes the formation of axonal filopodia and branches. However, the signaling and cytoskeletal mechanisms of
Sarah M Short et al.
The Journal of cell biology, 168(4), 643-653 (2005-02-18)
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Masayuki Yamashita
Communications biology, 6(1), 680-680 (2023-07-01)
Retinal ganglion cell (RGC) axons converge on the optic disc to form an optic nerve. However, the mechanism of RGC axon convergence remains elusive. In the embryonic retina, an electric field (EF) exists and this EF converges on the future
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