I2534
Anti-Importin β antibody,Mouse monoclonal
clone 31H4, purified from hybridoma cell culture
Synonyme(s) :
Anti-Imp β
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
31H4, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~97 kDa
Espèces réactives
rat, human, mouse, bovine
Technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa total cell extract
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KPNB1(3837)
mouse ... Kpnb1(16211)
rat ... Kpnb1(24917)
Description générale
Importin β comprises 19 tandemly repeated Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A), and the yeast kinase serine/threonine-protein kinase-TOR1 (HEAT) motifs and wraps around the importin-β (IBB). It also consists of an N-terminal RAN guanosine triphosphate (GTP) binding domain, a middle zone interacting with the nuclear pore, and an importin α binding domain at the C-terminus. The importin β gene is mapped to human chromosome location 17q21.32.
Monoclonal Anti-Importin b (mouse IgG1 isotype) is derived from the 31H4 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with importin. Importin β (Imp β), also referred to as karyopherin β1, is an import receptor that belongs to the family of nuclear transport receptors.
Spécificité
Monoclonal Anti-Importin β recognizes human, bovine, rat, and mouse importin β.
Immunogène
importin β
Application
Anti-Importin β antibody,Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- immunofluorescent staining
- coimmunoprecipitaion
- western blotting
- immunofluorescence
- immunoprecipitation
Actions biochimiques/physiologiques
Importin β (Impβ) forms a complex with importin α, which, in turn, binds the cargo protein via its NLS (nuclear localization signals). The cargo protein is transported by this ternary complex to the cytoplasmic face of the nuclear pore complex (NPC) via a direct interaction with specific nucleoporins. When the complex reaches the nuclear site of the nuclear pore complex (NPC), Ran-GTP binds to Impβ to form an Impβ/Ran-GTP complex and releases Impα and the cargo protein. The Impβ/Ran-GTP complex is then transported back to the cytoplasm, where the complex dissociates upon hydrolysis of GTP to GDP. Importin β is then ready to participate in new nuclear import cycle.
Importin β regulates cellular functions, such as mitotic spindle assembly and centrosome dynamics. It is implicated in the insertion of integral membrane proteins into the inner nuclear membrane. Importin β also functions as a chaperone for highly basic proteins in the cytoplasm.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Importin-beta-like nuclear transport receptors
Strom AC and Weis K
Genome Biology, 2(6) (2001)
Axoplasm isolation from peripheral nerve
Rishal I, et al.
Developmental neurobiology, 70(2), 126-133 (2010)
Subcellular knockout of importin beta1 perturbs axonal retrograde signaling
Perry RBT, et al.
Neuron, 75, 294-305 (2012)
R Truant et al.
Molecular and cellular biology, 19(2), 1210-1217 (1999-01-16)
Protein nuclear import is generally mediated by basic nuclear localization signals (NLSs) that serve as targets for the importin alpha (Imp alpha) NLS receptor. Imp alpha is in turn bound by importin beta (Imp beta), which targets the resultant protein
E Komlodi-Pasztor et al.
Oncogene, 28(35), 3111-3120 (2009-07-08)
Previous studies have described one nuclear localization signal (NLSI) in p53 and speculated on two additional sites termed NLSII and NLSIII. Drug-resistant KB cells selected with cisplatin or oxaliplatin were found to have increased p53 levels and in oxaliplatin-selected cells
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