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H7017

Sigma-Aldrich

Hemocyanin from Megathura crenulata (keyhole limpet)

Synonyme(s) :

KLH, Keyhole limpet hemocyanin

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About This Item

Numéro CAS:
9013-72-3
Numéro MDL:
MFCD00131280
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.61

Source biologique

Megathura crenulata

Niveau de qualité

300

Forme

powder

Conditionnement

vial of KLH (KLH is present by weight at approximately 1 part KLH to 5 parts total solid.)

Technique(s)

ELISA: suitable

Solubilité

H2O: soluble

Température de stockage

2-8°C

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Description générale

Gastropod hemocyanins are massive glycoproteins (4 to 8 MDa) designed by an intricate arrangement of 10 subunits that are self-assembled into hollow cylinders 35 nm in diameter.
Hemocyanin is a high molecular weight copper containing glycoprotein. It reversibly binds oxygen and forms the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, also called the giant keyhole limpet. It is native to the southern California coast and Mexico.

Application

Keyhole limpet hemocyanin (KLH) is a strong and well known immune-stimulant in both experimental animals and humans. It has been used as a hapten carrier protein to enhance antigen-specific T cell priming and is known to stimulate a CD4+ T-cell response. Hemocyanins stimulate Th1 reactions without known side effects, which make them ideal for long-term continuous treatment of cancer.

Forme physique

Lyophilized with stabilizing buffer

Reconstitution

Reconstitute with deionized water to a KLH concentration of 10 mg/mL, to yield an opalescent solution in 31 mM sodium phosphate buffer, pH 7.4, containing 0.46 M NaCl, 2% PVP and 41 mM sucrose.
Reconstituted protein solution may be stored up to 2 months at −20 °C.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Robert J Huber et al.
Histochemistry and cell biology, 136(2), 177-189 (2011-07-19)
The Dictyostelium discoideum homolog of mammalian cyclin dependent kinase 5 (Cdk5) has previously been shown to be required for optimal growth and differentiation in this model organism, however, the subcellular localization of the protein has not previously been studied. In
Chloe C Josefson et al.
Integrative and comparative biology, 60(3), 732-741 (2020-08-21)
One of the key foci of ecoimmunology is understanding the physiological interactions between reproduction and immune defense. To assess an immune challenge, investigators typically measure an immune response at a predetermined time point that was selected to represent a peak
J T Brisbin et al.
Poultry science, 87(10), 1995-1999 (2008-09-24)
Subtherapeutic and prophylactic doses of virginiamycin are capable of altering the intestinal microbiota as well as increasing several growth parameters in chickens. In spite of the fact that the microbiota plays a role in shaping the host's immune system, little
Jing Xing et al.
Fish & shellfish immunology, 66, 78-85 (2017-05-10)
In acquired immunity, T lymphocytes regulate the immune responses of B lymphocytes, including the IgM
Tingting Chen et al.
Cancer biology & medicine, 17(1), 132-141 (2020-04-17)
Objective: Cancer stem cell is one of the important causes of tumorigenesis as well as a drug target in the treatment of malignant tumor. However, at present, there is no immune vaccine targeting these cells. Octamer-binding transcription factor 4 (OCT4)
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