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GE17-5181-01

Sigma-Aldrich

SOURCE 15Q 4.6/100 PE

Synonyme(s) :

SOURCE 15Q Column

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

ligand

quaternary ammonium

Matériaux

polypropylene column

Description

Ion Exchanger Type (value)

Conditionnement

pkg of 1 ea

Paramètres

580 psi

Taille du lit

4.6 mm × 100 mm

Volume du lit

1.7 mL

D.I. de la colonne

4.6 mm

Matrice

polymeric
polystyrene/divinylbenzene

Groupe de la matrice active

ammonium ion, quaternary phase

Taille moy. des particules

15 μm

cleaning

1-14

working range

2-12

Adéquation

suitable for bioprocess medium

Technique de séparation

anion exchange

Catégories apparentées

Description générale

SOURCE 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

Caractéristiques et avantages

  • SOURCE 15Q media is highly suitable for fast, high-resolution purifications and easy scale-up
  • Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
  • Improved capacity compared to MonoBeads but with a slightly lower resolution.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

SOURCE is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocoles

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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