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G5768

Sigma-Aldrich

Gamborg′s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

Synonyme(s) :

B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.72

Forme

fine powder

Niveau de qualité

200

Technique(s)

cell culture | plant: suitable

Application(s)

agriculture

Température de stockage

2-8°C

Catégories apparentées

Description générale

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Application

Gamborg′s B-5 Basal Salt Mixture has been used:
  • as a constituent during the preparation of TEX buffer
  • as a media to maintain potato suspension and callus cultures
  • as a media in the primary-node transformation method

Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Variante de formule

With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation

Quantité

Formulated to contain 3.1 grams of powder per liter of medium.

Pictogrammes

Flame over circle
GHS03

Mention d'avertissement

Warning

Mentions de danger

H272

Classification des risques

Ox. Sol. 3

Code de la classe de stockage

5.1B - Oxidizing hazardous materials

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000420644
0000368108
0000346823
0000346823
0000368108

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Li-Da Wang et al.
Journal of Asian natural products research, 4(3), 171-174 (2002-07-18)
Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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