F2145

Monoclonal Anti-Factor V antibody produced in mouse

clone HV-1, purified immunoglobulin, buffered aqueous solution

• Numéro MDL: MFCD00164642
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: HV-1, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human
• Technique(s): ELISA: suitable; dot blot: suitable; neutralization: suitable
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... F5(2153)

Description

Description générale

Monoclonal Anti-Factor V (mouse IgG1 isotype) is derived from the HV-1 hybridoma, produced by the fusion of mouse myeloma Sp2/0-Ag14 cells and splenocytes from BALB/c mouse immunized with Factor V, purified from human plasma. Blood coagulation factor V, also known as, proaccelerin or labile factor. The activated factor V (factor Va) is a calcium dependent heterodimer composed of heavy chain (~105 kDa) and light chain (~73 kDa).

Spécificité

The antibody inhibits the activity of Factor V as measured by the prothrombin time (PT) assay.

Immunogène

Factor V from pooled normal human plasma

Application

Monoclonal Anti-Factor V antibody produced in mouse has been used in dot blot and enzyme linked immunosorbent assay (ELISA).

Actions biochimiques/physiologiques

Blood coagulation factor V is an essential component of the prothrombinase complex that catalyzes the rapid conversion of prothrombin to thrombin and is involved in intrinsic and extrinsic coagulation pathways. About 20% of blood factor V is contained within platelets. Factor V circulates in the plasma as a single chain glycoprotein with little or no intrinsic procoagulant activity. It is activated through limited proteolysis by thrombin, factor Xa, or the factor V activator from Russell′s viper venom (RW-V). Activated protein C (APC) with its cofactor protein S, inactivates factors Va and VIIIa to provide a major natural anticoagulant system. APC resistance known as factor V Leiden (FV Leiden) is a common hereditary thrombophilia Factor V mutation, caused by arginine substitution into glutamine at position 506 (R506Q).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable